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SOLVED: Complete this master mix table for 5 DNA samples. When deciding how many reactions to calculate, be sure to include the necessary controls required to interpret the PCR result relative to
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BioInformatics - PCR Efficiency in real-time PCR
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Gene Quantification & real time PCR quantification strategy
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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